Allelic variations of LcyE and crtRB1 genes in tropical adapted yellow endosperm maize inbred lines
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Date
2013-09
Authors
Adeyemo, O. A.
Omidiji, O.
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Society of Genetics & Plant Breeding
Abstract
Maize is an important staple cereal crop in sub-Saharan
Africa. Tropical-adapted yellow endosperm maize varieties
in Africa have low amounts of pro-vitamin A. Use of
molecular markers that detect alleles associated with high
level of pro-vitamin A can help to improve the pro-vitamin
A content in maize through conventional breeding. In this
study polymerase chain reaction (PCR) based markers were
used to characterize two sets of tropical-adapted yellow
endosperm maize inbred lines for allelic variants, present
in two carotenoid biosynthesis genes encoding lycopene
epsilon cyclase (LcyE) and -carotene hydroxylase 1
(crtRB1). Frequency of the favourable allele at LcyE 3’TE
polymorphism is 29% in Set 1 and 67% in Set 2, and the
5TE was present at frequencies of 24% in S1 and 3.3% in 2.
The frequency of the favourable allele at the LcyE SNP216
polymorphism was more than 95% in both the sets of inbred
lines. None of the lines included in Set 1 had favourable
alleles for crtRB1. On the other hand, in Set 2 the favourable
allele in the crtRB1 5TE insertion, had a frequency of 14%
whereas the favourable allele-frequency was 11% for crtRB1
3TE and was 1% for InDel4. Twelve maize inbred lines (9.8%)
had favourable alleles for crtRB1 3’TE and 5TE in Set 2.
None of the maize inbred lines carried favourable alleles for
all three crtRB1 polymorphisms. Favourable haplotypes of
LcyE and crtRB1 were found together in fifteen (12%) inbred
lines.
Description
Keywords
Maize inbred lines, allele specific markers, B-carotene, pro-vitamin A
Citation
Adeyemo, O and Omidiji, O. 2013. Allelic variations of LcyE and crtRB1 genes in tropical adapted yellow endosperm maize inbred lines. Indian J. Genet. 73(4): 363-370.