Relationship within and between Solanum L. Species Based on SDS-Polyacrylamide Gel Electrophoresis of Seed Proteins.
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Date
2003
Authors
Oboh, B.O.
Ajao, S. K.
Journal Title
Journal ISSN
Volume Title
Publisher
Bio-Science Research Bulletin.
Abstract
The relationship within and between 20 cultivars of five Solanum L species was investigated. Results from the SDS-polyacrylamide gel electrophoresis of the seed proteins revealed 47 polypeptide bands with molecular weight from 2.5 to 105.5 kilodaltons (kD). The cultivars could be characterized by the presence and or absence, staining intensities (degree of manifestation of proteins) and electrophoretic mobilities of the bands. The· bands were assigned to 3 zones based on the electrophoretic mobilities - fast. intermediate and slow moving band with majority of the bands in the slow moving zone. There was a common inter and intra-specific band at 3.5cm (44.5KD). There was no correlation between the banding pattern and the shape or colour of the fruit, Similarity indices shows that cultivars within the same species are more closely related than the ones between species. Data presented shows that SDS-polyacrylamide gel electrophoresis of seed storage proteins is useful in classifying Solanum germplasm and that it helps in establishing the relationship within and between Solanum species
Description
Keywords
Solanum species , Seed proteins , Polyacrylamide gel electrophoresis , Cultivars , Polypeptides , Genetic diversity , Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics
Citation
Oboh, B.O. & Ajao, S. K. (2003). Relationship within and between Solanum L. Species Based on SDS-Polyacrylamide Gel Electrophoresis of Seed Proteins. Bio-Science Research Bulletin 19(2), 179-188.