Analyses of antisickling potency of Carica papaya dried leaf extract and fractions
Date
2010-11
Authors
Imaga, Ngozi Awa
Adepoju, Olusegun A.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Carica papaya dried leaves have been indicated in sickle cell anemia management by local indigenous
folk and in recent scientific research. In this research, dried C. papaya leaves were extracted using the
soxhlet extraction method with 5 different solvents to give five different fractions namely hexane,
chloroform, ethyl acetate, butanol and water. This research examined the crude extract and the various
leaf extract fractions of C. papaya L. (Caricaceae) for possible in vitro antisickling activities on Hbss red
blood cells obtained from non-crisis state sickle cell patients involving the use of positive (phydroxybenzoic
acid 5 mg /ml) and negative (normal saline) controls for the antisickling experiments.
Pretreatment of SS cell suspensions with C. papaya leaf extract and fractions all inhibited formation of
sickle cells under severe hypoxia at varying degrees, with only 0 - 5% sickle cells in the crude extract at
60 min compared with untreated SS cell suspensions which had over 80% sickle cells. Analysis of two
different concentrations of C. papaya crude extract (10 and 5 mg/ml) showed the 10 mg/ml extract as
the concentration with highest antisickling effect. Butanol extract showed the highest antisickling
activity at 10 mg/ml concentration, while the ethyl acetate extract had the highest antisickling activity at
5 mg/ml concentration. These results further indicate the possibility of C. papaya leaf extract as
potential phytotherapy for sickle cell anemia.
Description
Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , Antisickling , sickle cell disorder , C. papaya , fractionation
Citation
Ngozi Awa Imaga and Olusegun A. Adepoju Journal of Pharmacognosy and Phytotherapy. 2(7) 97-102. 2010