Genetic Polymorphisms of Glucose-6-Phosphate Dehydrogenase in Lagos, Nigeria
Date
2018-03-01
Authors
Babalola, Musa O.
Imaga, Ngozi A.
Samuel, Titilola A.
Diriwari, Iyanu P.
Kolade, Olajumoke
Ezeamalu, Irene
Laoye, Adefioye O.
Ojewunmi, Oyesola O.
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis
Abstract
Glucose-6-phosphate dehydrogenase (G6PD) is an essential enzyme in the pentose phosphate pathway
that prevents oxidative damage to cells. This study determined the genotypic and allelic frequencies of
G6PD G202A and A376G and also investigated correlation between G6PD polymorphisms and hemoglobin
(Hb) phenotypes in children in Lagos, Nigeria. Seventy-eight children [55 with Hb AA (bA/bA) and
23 with Hb AS (bA/bS) trait] and 65 Hb SS (bS/bS) (HBB: c.20A>T) subjects in steady state with age range
between 5–15 years were recruited for the study. Hemoglobin phenotypes of all study participants
were carried out using alkaline electrophoresis and solubility tests. Genomic DNA was extracted from
whole blood and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was
used to determine the G202A and the A376G mutations of the G6PD gene. The genotype and allele distributions
of G6PD G202A and A376G according to the Hb phenotypes were not statistically significant
(p>0.05). The minor allele frequency 202A was 0.15 (15.0%) and 0.14 (14.0%) in cases and controls,
respectively. The overall frequency of 376G allele in the case group was 0.35 (35.0%) and 0.38 (38.0%)
in the control group. No statistical significance was observed in the genotype and allele distributions of
A376G in both the case and control groups (p>0.05). The G6PD A frequency in Hb SS subjects and
the control group were 6.2 and 2.6%, respectively. G6PD G202A and A376G polymorphisms were not
associated with Hb phenotypes and the allele distributions of 202A and 376G in this study are typical
of West African populations.
Description
Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , Genetic polymorphism , glucose-6-phosphate dehydrogenase (G6PD) , Nigeria , sickle cell anemia
Citation
Musa O. Babalola, Ngozi A. Imaga, Titilola A. Samuel, Iyanu P. Diriwari, Olajumoke Kolade, Irene Ezeamalu, Adefioye O. Laoye & Oyesola O. Ojewunmi (2018): Hemoglobin, DOI:10.1080/03630269.2018.1434196