Modification of pcr ribotyping method for application as a routine typing scheme for clostridium difficile

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O'neil, G.L.
Ogunsola, F.T.
Brazier, J.S.
Duerden, B.I.
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Clinically significant jsolates of Gram-negative bacilli obtained in the Lagos University Teaching Hospital Laboratory between January 1995 and June 1996 were characterised, using Analytical profile index system (APl 0 20E and API 20NE), and also by simple conventional laboratory methods. Twenty-lwo percent (163) of all clinical signifi· cant isolates were found to be non-fermenting Gram-negative bacilli. The micro-organisms identi· fied were Pseudomonas aeruginosa (55%), Acinetobacter species (29.5%). Burkholderia (Pseudononas) cepacia (9%), Pseudomonas tluorescens (2%), Pseudononas stutzeti (1 %), Stenotrophomonas (Xanthomonas) maltophi/a (0.6%), .. Chryseomonas luteola (0.6%), Ochrobacterium anthropi (0.6%), Agrobacterium radiobacter (0.6%) and Alcaligenes xylosoxidans (0.6%). Seventy percent of the isolates were obtained from specimens from hospitalised patients while 30�. were obtained from specimens from outpatient clinics. Most of the non-fermenting Gram-negative bacterial isolates were obtained from wound swabs (39%) and urinary specimens (36%). Nine percent was obtained from pulmonary specimens, 4.5% frorn eye swabs, 4.5% from ear swabs and 5.5% frorn blood. Very few (1.5%) isolates were cultured frorn the cerebrospinal fluid.
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Characterization , Gram-negative bacili , Non-fermentative. , Lagos University Teaching Hospital , Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases
O'Neill, G.L, Ogunsola, F.T, Brazier, J.S. and Duerden, B.I. (1996). Modification of a Polymerase Chain Reaction-ribotyping method for application as a routine typing scheme for Clostridium difficile. Anaerobe 2: 205-209.