Modification of pcr ribotyping method for application as a routine typing scheme for clostridium difficile
Loading...
Date
1996
Authors
O'neil, G.L.
Ogunsola, F.T.
Brazier, J.S.
Duerden, B.I.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Clinically significant jsolates of Gram-negative bacilli obtained in the Lagos University Teaching Hospital Laboratory between January 1995 and June 1996 were characterised, using Analytical profile index system (APl 0 20E and API 20NE), and also by simple conventional laboratory methods. Twenty-lwo percent (163) of all clinical signifi· cant isolates were found to be non-fermenting Gram-negative bacilli. The micro-organisms identi· fied were Pseudomonas aeruginosa (55%), Acinetobacter species (29.5%). Burkholderia (Pseudononas) cepacia (9%), Pseudomonas tluorescens (2%), Pseudononas stutzeti (1 %), Stenotrophomonas (Xanthomonas) maltophi/a (0.6%), .. Chryseomonas luteola (0.6%), Ochrobacterium anthropi (0.6%), Agrobacterium radiobacter (0.6%) and Alcaligenes xylosoxidans (0.6%). Seventy percent of the isolates were obtained from specimens from hospitalised patients while 30�. were obtained from specimens from outpatient clinics. Most of the non-fermenting Gram-negative bacterial isolates were obtained from wound swabs (39%) and urinary specimens (36%). Nine percent was obtained from pulmonary specimens, 4.5% frorn eye swabs, 4.5% from ear swabs and 5.5% frorn blood. Very few (1.5%) isolates were cultured frorn the cerebrospinal fluid.
Description
Staff publications
Keywords
Characterization , Gram-negative bacili , Non-fermentative. , Lagos University Teaching Hospital , Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases
Citation
O'Neill, G.L, Ogunsola, F.T, Brazier, J.S. and Duerden, B.I. (1996). Modification of a Polymerase Chain Reaction-ribotyping method for application as a routine typing scheme for Clostridium difficile. Anaerobe 2: 205-209.
URI
https://www.google.com/search?q=Characterization+of+Non-Fermenting+Gram-Negative+Bacilli+at+the+D+Lagos+University+Teaching+Hospital+-+A+Preliminary+Report.&oq=Characterization+of+Non-Fermenting+Gram-Negative+Bacilli+at+the+D+Lagos+University+Teaching+Hospital+-+A+Preliminary+Report.&aqs=chrome..69i57.516j0j9&sourceid=chrome&ie=UTF-8
https://ir.unilag.edu.ng/handle/123456789/7273
https://ir.unilag.edu.ng/handle/123456789/7273