Modification of pcr ribotyping method for application as a routine typing scheme for clostridium difficile

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dc.contributor.authorO'neil, G.L.
dc.contributor.authorOgunsola, F.T.
dc.contributor.authorBrazier, J.S.
dc.contributor.authorDuerden, B.I.
dc.date.accessioned2020-01-06T13:34:03Z
dc.date.available2020-01-06T13:34:03Z
dc.date.issued1996
dc.descriptionStaff publicationsen_US
dc.description.abstractClinically significant jsolates of Gram-negative bacilli obtained in the Lagos University Teaching Hospital Laboratory between January 1995 and June 1996 were characterised, using Analytical profile index system (APl 0 20E and API 20NE), and also by simple conventional laboratory methods. Twenty-lwo percent (163) of all clinical signifi· cant isolates were found to be non-fermenting Gram-negative bacilli. The micro-organisms identi· fied were Pseudomonas aeruginosa (55%), Acinetobacter species (29.5%). Burkholderia (Pseudononas) cepacia (9%), Pseudomonas tluorescens (2%), Pseudononas stutzeti (1 %), Stenotrophomonas (Xanthomonas) maltophi/a (0.6%), .. Chryseomonas luteola (0.6%), Ochrobacterium anthropi (0.6%), Agrobacterium radiobacter (0.6%) and Alcaligenes xylosoxidans (0.6%). Seventy percent of the isolates were obtained from specimens from hospitalised patients while 30�. were obtained from specimens from outpatient clinics. Most of the non-fermenting Gram-negative bacterial isolates were obtained from wound swabs (39%) and urinary specimens (36%). Nine percent was obtained from pulmonary specimens, 4.5% frorn eye swabs, 4.5% from ear swabs and 5.5% frorn blood. Very few (1.5%) isolates were cultured frorn the cerebrospinal fluid.en_US
dc.identifier.citationO'Neill, G.L, Ogunsola, F.T, Brazier, J.S. and Duerden, B.I. (1996). Modification of a Polymerase Chain Reaction-ribotyping method for application as a routine typing scheme for Clostridium difficile. Anaerobe 2: 205-209.en_US
dc.identifier.urihttps://www.google.com/search?q=Characterization+of+Non-Fermenting+Gram-Negative+Bacilli+at+the+D+Lagos+University+Teaching+Hospital+-+A+Preliminary+Report.&oq=Characterization+of+Non-Fermenting+Gram-Negative+Bacilli+at+the+D+Lagos+University+Teaching+Hospital+-+A+Preliminary+Report.&aqs=chrome..69i57.516j0j9&sourceid=chrome&ie=UTF-8
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7273
dc.language.isoenen_US
dc.subjectCharacterizationen_US
dc.subjectGram-negative bacilien_US
dc.subjectNon-fermentative.en_US
dc.subjectLagos University Teaching Hospitalen_US
dc.subjectResearch Subject Categories::MEDICINE::Microbiology, immunology, infectious diseasesen_US
dc.titleModification of pcr ribotyping method for application as a routine typing scheme for clostridium difficileen_US
dc.typeArticleen_US
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