Amplification of COI-II, ND II & ITSI-II by Polymerase Chain Reaction: Prospects for DNA Barcoding of Nigerian Drosophila Populations
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KLOBEX Journal of the Nigerian Society of Experimental Biology
Populations of Nigerian Drosophila are hardly distinguishable in morphology but reproductively isolated. Spectrophotometric analysis of genomic DNA of the fruitflies reveals that the DNA extraction protocol used yielded genomic DNA good enough for polymerase chain reaction, confirming claims that polymerase chain reaction is possible even when amount of template DNA varies considerably. The mitochondrial cytochrome oxidase subunits I and II, nicotinamide adenine dinucleotide dehydrogenase subunit II, and the nuclear internal transcribed spacer regions I and II are highly conserved regions in Drosophila. Successful amplification of these regions suggests that they are promising regions for DNA barcoding and molecular characterization of local Drosophila species. Further molecular and DNA barcoding studies targeted at local Drosophila species in Nigeria is desirable.
Nigeria , Drosophila , ND 2 , COI , COII , ITSI , ITSII
Adekoya, K. O.and Ogundele, E.O. (2013). Amplification of COI-II, ND II & ITSI-II by Polymerase Chain Reaction: Prospects for DNA Barcoding of Nigerian Drosophila Populations. NISEB Journal, 13 (1 & 2): 73 – 77.