Amplification of COI-II, ND II & ITSI-II by Polymerase Chain Reaction: Prospects for DNA Barcoding of Nigerian Drosophila Populations

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dc.contributor.authorAdekoya, K.O.
dc.contributor.authorOgundele, O.E.
dc.date.accessioned2019-01-17T07:55:06Z
dc.date.available2019-01-17T07:55:06Z
dc.date.issued2013-07
dc.description.abstractPopulations of Nigerian Drosophila are hardly distinguishable in morphology but reproductively isolated. Spectrophotometric analysis of genomic DNA of the fruitflies reveals that the DNA extraction protocol used yielded genomic DNA good enough for polymerase chain reaction, confirming claims that polymerase chain reaction is possible even when amount of template DNA varies considerably. The mitochondrial cytochrome oxidase subunits I and II, nicotinamide adenine dinucleotide dehydrogenase subunit II, and the nuclear internal transcribed spacer regions I and II are highly conserved regions in Drosophila. Successful amplification of these regions suggests that they are promising regions for DNA barcoding and molecular characterization of local Drosophila species. Further molecular and DNA barcoding studies targeted at local Drosophila species in Nigeria is desirable.en_US
dc.description.sponsorshipNISEB JOURNALen_US
dc.identifier.citationAdekoya, K. O.and Ogundele, E.O. (2013). Amplification of COI-II, ND II & ITSI-II by Polymerase Chain Reaction: Prospects for DNA Barcoding of Nigerian Drosophila Populations. NISEB Journal, 13 (1 & 2): 73 – 77.en_US
dc.identifier.issn1595 - 6938
dc.identifier.urihttp://ir.unilag.edu.ng:8080/handle/123456789/3520
dc.language.isoenen_US
dc.publisherKLOBEX Journal of the Nigerian Society of Experimental Biologyen_US
dc.subjectNigeriaen_US
dc.subjectDrosophilaen_US
dc.subjectND 2en_US
dc.subjectCOIen_US
dc.subjectCOIIen_US
dc.subjectITSIen_US
dc.subjectITSIIen_US
dc.titleAmplification of COI-II, ND II & ITSI-II by Polymerase Chain Reaction: Prospects for DNA Barcoding of Nigerian Drosophila Populationsen_US
dc.typeArticleen_US
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