Postmortem Time Affects Brain, Liver, Kidney and Heart DNA in Male Rat
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Date
2015
Authors
Ebuehi, O.A.T.
Motolani Amode
Ayooluwa Balogun
Adenike Fowora
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American Journal of Biochemistry
Abstract
The aim of this paper is to evaluate the effect of post-mortem time (0, 24, 48 h) on the integrity of DNA vextracted from the brain, liver, heart and kidney of male albino Sprague –Dawley rats. Random Amplification of Polymorphic DNA–Polymerase Chain Reaction (RAPD-PCR) followed by agarose electrophoresis were used to detect the correlation between DNA integrity and post mortem time. The results of the post-mortem DNA profile showed that DNA degradation was a time dependent process. DNA from brain, as compared to liver and kidney DNA, showed a slower degradation rate and therefore brain could serve as a valuable organ for studying degradation in longer post-mortem time. This study provides the first DNA profile analysis of the postmortem progress of DNA degradation in rats.
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Ebuehi, O.A.T, Motolani Amode, Ayooluwa Balogun, Adenike Fowora (2015). Postmortem Time Affects Brain, Liver, Kidney and Heart DNA in Male Rat. American Journal of Biochemistry 5(1): 1-5