Detection of fus-1 (OXA-85), a class d betalactamase from fusobacterium nucleatum subspecies polymorphum in Nigeria

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Nwaokorie, F.O.
Savage, K.O.
Fowora, M.A.
Ayanbadejo, P.O.
Ogunsola, F.T.
Coker, A.O.
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Aims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene. Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010. Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii. Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum. Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.
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Beta-lactamase , Resistance genes , Fusobacterium nucleatum , Subspecies polymorphum , Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases
Nwaokorie, F.O, Savage, K.O, Fowora, M.A, Ayanbadejo, P.O, Ogunsola, F.T and, Coker, A.O. (2013). Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum subspecies polymorphum in Nigeria. British Microbiology Research Journal; 3: 492-500