Detection of fus-1 (OXA-85), a class d betalactamase from fusobacterium nucleatum subspecies polymorphum in Nigeria

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dc.contributor.authorNwaokorie, F.O.
dc.contributor.authorSavage, K.O.
dc.contributor.authorFowora, M.A.
dc.contributor.authorAyanbadejo, P.O.
dc.contributor.authorOgunsola, F.T.
dc.contributor.authorCoker, A.O.
dc.date.accessioned2020-01-13T13:57:25Z
dc.date.available2020-01-13T13:57:25Z
dc.date.issued2013
dc.descriptionStaff publicationsen_US
dc.description.abstractAims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene. Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010. Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii. Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum. Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.en_US
dc.identifier.citationNwaokorie, F.O, Savage, K.O, Fowora, M.A, Ayanbadejo, P.O, Ogunsola, F.T and, Coker, A.O. (2013). Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum subspecies polymorphum in Nigeria. British Microbiology Research Journal; 3: 492-500en_US
dc.identifier.urihttps://www.google.com/search?q=Detection+of+FUS-1+(OXA-85)%2C+a+Class+D+Betalactamase+from+Fusobacterium+nucleatum+Subspecies+Polymorphum+in+Nigeria&oq=Detection+of+FUS-1+(OXA-85)%2C+a+Class+D+Betalactamase+from+Fusobacterium+nucleatum+Subspecies+Polymorphum+in+Nigeria&aqs=chrome..69i57j69i60.753j0j9&sourceid=chrome&ie=UTF-8
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7356
dc.language.isoenen_US
dc.relation.ispartofseriesBritish Microbiology Research Journal;Vol.3
dc.subjectBeta-lactamaseen_US
dc.subjectResistance genesen_US
dc.subjectFusobacterium nucleatumen_US
dc.subjectSubspecies polymorphumen_US
dc.subjectResearch Subject Categories::MEDICINE::Microbiology, immunology, infectious diseasesen_US
dc.titleDetection of fus-1 (OXA-85), a class d betalactamase from fusobacterium nucleatum subspecies polymorphum in Nigeriaen_US
dc.typeArticleen_US
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