Sequence Variability in the NRAMP1 Gene among Indigenous and Exotic Chicken Types
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Date
2019-06-18
Authors
ABAZUH, U.D.
Adekoya, K.O.
Oboh, B.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Universitas Indonesia
Abstract
The natural resistance-associated macrophage protein 1 (NRAMP1) gene in chickens, which exists on chromosome 7, is
thought to play a significant role in disease resistance. Variations in this gene have been documented and have played
crucial roles in the variations in the resistance and/or susceptibility that is expressed by individuals and different groups
of animal species. In this study, the NRAMP1 gene was studied for single nucleotide polymorphism (SNP) variability
between indigenous and exotic chicken breeds. The gene was amplified using the polymerase chain reaction (PCR)
method and sequenced and analyzed. Six SNPs, both synonymous and non-synonymous (C3700T, G3702C, A3712C,
C3714G, C3693G, and G3705T), were detected in the gene in both indigenous and exotic chicken types. One allele
form was detected among all the sample animals that were studied. A phylogenetic tree revealed that the indigenous
chicken type and the exotic broiler chicken type are genetically similar with respect to this gene. However, the exotic
layer chicken type is genetically suggested to be distantly related to both the indigenous and broiler chicken types, indicating that the gene has probably been evolving both within and among different poultry species.
Description
Keywords
Chicken , NRAMP1 gene , nucleotide variation , phylogenic tree
Citation
Abazuh, Uchenna Desmond, Adekoya, Khalid Olajide and Oboh, Bola Olufunmilayo (2019). Sequence Variability in the NRAMP1 Gene Among Indigenous and Exotic Chicken Types. Makara Journal of Science. 23 (2) : 103 - 108.