Department of Anatomy

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Browsing Department of Anatomy by Author "Ama, C"

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  • Item
    Open Access
    D-Ribose-L-Cysteine-rich supplement attenuates doxorubicin-induced impaired Spermatogenesis, testicular steroidogenesis and redox status in Sprague-Dawley rats
    (Afr. J. Biomed. Res., 2019) Medubi, L.J; Ama, C; Nwosu, N.C; Lawal, O.R; Osinubi, A.A.A
    Reproductive function is often impaired during chemotherapy and currently there is no universally approved drug/supplement that can effectively protect the testis from cellular assault due to chemotherapeutic agent such as doxorubicin (dox). This study was designed to investigate the efficacy of D-Ribose-L-Cysteine rich supplement, CellgevityTM (cgt), in ameliorating some doxorubicin-induced testicular damage in Sprague-Dawley rats. At the end of the study, biochemical assays and sperm epididymal parameters analysis were done. Our results show that simultaneous administration of cgt and doxorubicin has ameliorative effect on the testis when compared to pre-treatment or post-treatment with cgt. Significant increase in testosterone, sperm concentration, progressive motility and significant decrease in immotility were observed in the cellgevity treated group. Also, simultaneous administration of cgt with dox reduced lipid peroxidation and boosted only glutathione level in testicular tissues. Nevertheless, pre-treatment or post-treatment with cgt did not play a significant role in modulating sperm parameters, hormones and tissue oxidative stress level. This result reveals that cellgevity has testiculo-protective effect against doxorubicin administration.
  • Item
    Open Access
    Increased de novo glutathione production enhances sexual dysfunctions in rats subjected to paradoxical sleep deprivation
    (JBRA Assisted Reproduction, 2021) Medubi, L.J; Nwosu, N.C; Medubi, O.O; Lawal, O.R; Ama, C; Kusemiju, T.O; Osinubi, A.A
    Objective: Poor quality of sexual life has been report- ed secondary to poor sleep or sleep deprivation. Paradoxi- cal sleep is an integral part of the sleep-wakefulness phys- iology and prolonged paradoxical sleep deprivation (PSD) may even be fatal. The objective of this investigation was to determine if D-ribose-L-cysteine (RibCys) and zinc (Zn) administration can attenuate the effect of PSD on the sex- ual function of male rats. Methods: Following acclimatization, 25 male rats were randomly distributed into five groups of 5 rats each. The PSD, PSD+RibCys, PSD+Zn, PSD+RibCys+Zn, and Control groups were sleep-deprived only, sleep-deprived and giv- en 100mg/kg body-weight of pure RibCys, sleep-deprived and given 10mg/kg body weight Zn, sleep-deprived and given a combination of 100mg/kg of RibCys and 10mg/kg of Zn, and given distilled water without sleep deprivation, respectively. PSD lasted for 20 hours per day for 14 days. Subsequently, the sexual behavioral study was carried out and the animals were sacrificed for biochemical assays. Results: Analyses of results show that for animals treated with RibCys or Zn, all sexual parameters such as mount frequency and latency, intromission frequency and latency and ejaculation frequency and latency were signifi- cantly improved compared with animals subjected to PSD only. This improvement correlates strongly with serum glu- tathione (GHS) levels. Conclusion: In summary, riboceine increases circulat- ing GHS, which leads to improved sexual function during sleep deprivation.