Faculty of Pharmacy

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Browsing Faculty of Pharmacy by Subject "[1-(3-(Trifluoromethyl)benzyl]-piperazine"

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    Open Access
    Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species
    (Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria., 2017-11) Adeluola, A.O.; Oyedeji, K.S.; Mendie, U.E.; Johnson, J.R.; Porter, J.R.
    Purpose: To detect efflux pump activity (EPA) and screening a suspected efflux pump inhibitor (EPI) [1(3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could help in reducing multi-drug resistance (MDR). Methods: Eighteen isolates, viz, 14 S. aureus, 2 S. lentus, 1 S. xylosus and 1 Micrococcus species from various hospital infections of admitted patients were screened for antibiotics susceptibility to 11 classes of antibiotics including oxacillin and β-lactamase production. Efflux pump activity (EPA) was determined by minimum inhibitory concentration (MIC) technique in the presence and absence of TFMBP, the isolates were also screened for MDR genes. Results: All the isolates were resistant to ampicillin (10 µg) and penicillin (10 µg), but sensitive to bacitracin (10 µg). Majority of the isolates were MDR 12/18 (66.7 %), 10 (55.6 %) were inducible βlactamase producers and 3 (16.7 %) were intrinsic β-lactamase producers. Seven (38.9 %) were resistant to oxacillin and also produced carbapenemase enzyme. Eight (66.7 %) of the 12 MDR isolates gave evidence of EPA with TFMBP. However, no MDR genes were detected. Conclusion: Staphylococcus and Micrococcus species exhibit EPA in antibiotic resistance while a suitable EPI such as TFMBP when combined with specific antibiotics could help combat this menace.