Iridoid Glycoside from the Leaves of Clerodendrum volubile Beauv. Shows Potent Antioxidant Activity Against Oxidative Stress in Rat Brain and Hepatic Tissues
Date
2013-09-10
Authors
Ebuehi, O.A.T.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Dietary Supplements
Abstract
ABSTRACT. Aim: This study aims at reporting the isolation, structure elucidation,
and antioxidant potentials of ajugoside from C. volubile leaves in sodium nitroprusside
(SNP)-induced oxidative stressed rat brain and hepatic tissues. Materials and Method:
An iridoid monoterpene, ajugoside was isolated from the n-butanol fraction of C.
volubile and evaluated for its antioxidant protective potential on brain and liver tissues
of male Wister rats in an ex vivo model. Two molar concentrations (6.4 × 10−4 M and
1.28 × 10−3 M) of the metabolite and SNP were incubated with the tissues homogenate
at 37◦C for 2 hr prior to the test and assayed for catalase, superoxide dismutase (SOD)
activities, and lipid peroxidation. α tocopherol (6.4 × 10−4 M) was used as standard.
Results: Both molar concentrations exhibited high catalase activity in the tissues. How ever, 6.4 × 10−4 M ajugoside exhibited a very high SOD activity (liver: 96.45 and brain:
96.30%) and inhibition of lipid peroxidation (liver: 88.11 and brain: 93.27%) compared
to the standard. 1.28 × 10−3 M ajugoside also exhibited good activities but lower than
that of the standard and 6.4 × 10−4 M ajugoside. Discussion and Conclusion: Ajugoside
showed potent antioxidant activities as evidenced by the synergistic high activities of
SOD and catalase as well as inhibition of lipid peroxidation in the studied tissues.
Description
Keywords
Ajugoside, antioxidant, ex vivo, oxidative stress
Citation
Ochuko L. Erukainure, Osaretin A.T. Ebuehi, Iqbal M. Choudhary, Achyut Adhikari, Rahman M. Hafizur, Shahida Perveen, Aliyu Muhammad, & Gloria N. Elemo