ALLELE FREQUENCIES AND HAPLOTYPE DIVERSITIES OF FIVE Y - CHROMOSOME SHORT TANDEM REPEAT LOCI IN A RANDOM SAMPLE OF YORUBA POPULATION IN LAGOS, NIGERIA
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Date
2019-08
Authors
Fakorede, S.T.
Adekoya, K.O.
Akpan, U.U.
Ogunlusi, O.V.
Journal Title
Journal ISSN
Volume Title
Publisher
Federal University Wukari, Nigeria
Abstract
Y-chromosome short tandem repeats (Y-STRs) are specialized class of short tandem repeats located on human Y
(male) chromosomes and are passed unchanged (barring a mutation) from one generation to the next. They are
widely used in population genetic studies, forensics, paternity and genealogical DNA testing. The non-recombining
nature of these loci makes them to have high discriminatory capacities among individuals of the same population
or ethnicity. This is because they are polymorphic and exhibit high mutation rates. For Y-STR data to be applicable
in forensics, knowledge of the haplotype frequencies in a geographic or ethnic substructure is required. DNA was
extracted from peripheral blood sample of 110 unrelated Yoruba males, who have consented to participate in the
research by signing the informed consent form, using conventional method. Five Y-STR markers (DYS392,
DYS393, DYS449, DYS490 and DYS576) were typed and the PCR products were resolved on 4% agarose
electrophoresis. Haplotype diversity was highest in DYS449 (0.840) and lowest in DYS392 (0.406). Also, the
power of discrimination was found to be highest in DYS449 and lowest in DYS392 with 85.9% and 40.7%,
respectively. The study revealed that the loci under study are suitable for use in identification, discrimination and
forensic applications in the Yoruba population in Nigeria.
Description
Keywords
Discrimination , Forensics , Genetic Diversity , Haplotypes , Tandem Repeats , Yoruba
Citation
Fakorede, Samson Taiwo, Adekoya, Khalid Olajide, Akpan, Utom-Obong Udom and Ogunlusi, Olajumoke Victoria (2019). Allele Frequencies and Haplotype Diversities of Five Y-Chromosome Short Tandem Repeat Loci in a Random Sample of Yoruba Population in Lagos, Nigeria. . FUW Trends in Science and Technology Journal. 4 (2) : 577 - 581.