Optimization of protocols for DNA extraction and RAPD analysis in West African fonio (Digitaria exilis and Digitaria iburua ) germplasm characterization
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Date
2005-12
Authors
Kuta, D.D.
Kwon-Ndung, E.
Dachi, S.
Bakare, O
Ogunkanmi, L.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Fonio is an important indigenous grain crop of West Africa, but the extent of genetic diversity in fonio,
its origin and phylogeny are not well understood. DNA markers allow precise characterization of plant
germplasm accessions, but there is no literature report of their use in fonio. This paper reports the
result of protocol optimization research for DNA isolation and RAPD analyses in fonio. High quality
DNA was successfully isolated and RAPD was effectively used to study genetic similarity among fonio
accessions. This result might stimulate the application of DNA markers to investigate the origin and
phylogeny of fonio in Africa.
Description
Keywords
Digitaria spp., DNA markers, RAPD, germplasm characterization, fonio.
Citation
Kuta, D.D. Kwon-Ndung, E. Dachi, S. Bakare, O Ogunkanmi, L.A. 2005. Optimization of protocols for DNA extraction and RAPD analysis in West African fonio (Digitaria exilis and Digitaria iburua ) germplasm characterization. African Journal of Biotechnology.4(12): 1368-1371