Gas Chromatography-Mass Spectrometry and Cytotoxicity of Securidaca longepedunculata (polygalaceae) Root Bark Extract
Date
2016-03
Authors
Lawal, R.A
Ozaslan, M.D
Odesanmi, O.S
Ebuehi, O.A.T.
Karagoz, I.D
Kilic, I.H
Journal Title
Journal ISSN
Volume Title
Publisher
Fountain Journal of Natural and Applied Sciences
Abstract
Securidaca longepedunculata is a savannah shrub found growing in tropical Africa. It is reputed
to have more than a hundred medicinal uses and is a major component of anticancer decoctions in
Nigeria. Despite its reported use as an anti-cancer plant, there is a dearth of information on the
anti-cancer potential and mechanism of its anticancer activity. The present study is to identify
the chemical constituents present in Securidaca longepedunculata by quantitative Gas
Chromatography-Mass Spectrometry (GC-MS) analysis and determine the cytotoxic effect of the
plant on Ehrlich ascites carcinoma cells. Secondary metabolites in the extract were predicted
using GC-MS. In vitro cytotoxic activity was determined using the trypan blue dye exclusion assay
by incubating Ehrlich ascites carcinoma cells with various concentrations of S. longepedunculata
aqueous extract. Analysis by GC-MS revealed the presence of the following compounds: bis (2-
ethylhexyl) phthalate (90.99%), 1-decanol (4.17%) and cyclododecane (1.86%), phenol, 2, 21
-
methylene bis (1.32%), cyclopenten-4-one, 3-hydroxy-1, 2, 3, 5, 51
-pentakis (trimethylsilyloxy)-
(0.89%) and phenol, 2, 4-bis (1, 1-dimethylethyl)(0.78%). Securidaca longepedunculata aqueous
extract (SL) was cytotoxic to Ehrlich ascites carcinoma cells in vitro. The IC50 of SL on EAC cells
was 67 µg/ml. The aqueous root-bark extract of S. longepedunculata contains bioactive agents
and was cytotoxic to Ehrlich ascites carcinoma cells in vitro.
Description
Keywords
phthalate , Ehrlich , Medicinal , Plants