Antisickling and toxicological profiles of leaf and stem of Parquetina nigrescens L.
Date
2010-04-18
Authors
Imaga, N.O.A.
Gbenle, G.O.
Okochi, V.I.
Adenekan, S.O.
Edeoghon, S.O.
Kehinde, M.O.
Bamiro, S.B.
Ajiboye, A.
Obinna, A.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Folk medicine reportedly uses Parquetina nigrescens L. (Asclepiadaceae) as a herbal remedy for the
management of sickle cell anemia. This study was carried out to screen the leaves and stem of P.
nigrescens for antisickling activity, erythrocyte membrane -stabilizing effects and any end organ
toxicity. Percentage reversal and inhibition of sickling parameters were analyzed on pre-sickled HbSS
blood cell suspensions using sodium metabisulphite solution as inducer and 5 mg/ml
parahydroxybenzoic acid and normal saline as positive and negative controls respectively. Effects of
the plant extracts on the erythrocyte were assessed using osmotic fragility and the toxicity profile done
via LD50 and sub-acute toxicity studies on graded concentrations of extract. Results show that P.
nigrescens has appreciable antisickling activity, has no toxic effect when administered at low
concentrations and protects the integrity of the erythrocyte membrane as evidenced in the fragiliogram
by the reduction in hemolysis of the Hbss cells.
Description
Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , Antisickling activity , membrane integrity , osmotic fragility , Parquetina nigrescens , sickle cell disease
Citation
N. O. A. Imaga, G. O. Gbenle, V. I. Okochi, S.O. Adenekan, S. O. Edeoghon, M. O. Kehinde, S. B. Bamiro., A. Ajiboye and A. Obinna J. of Medicinal Plants Research. 4(8) 639-643. 2010