Methylene tetrahydrofolate reductase and methionine synthase gene polymorphisms as genetic determinants of pre-eclampsia.

Abstract
BACKGROUND:Pre-eclampsia(PE)isaleadingcauseofmaternalandneonatalmortalityinAfrica;andhasbeen associatedwiththeinterplayofgenetic,metabolicandenvironmentalfactors.Polymorphismsofmethylenetetrahydrofolatereductase(MTHFR)andmethioninesynthase(MTR)folatecyclegenes,havebeencon-troversiallyassociatedwithpre-eclampsiainstudiesfromdifferenthumanpopulations.Objectives: To determine the distribution of MTHFR C677T and MTR A2756G polymorphisms in a Nigerian population and evaluate possible associations with the occurrence of pre-eclampsia and homocysteine metabolic derangement.MATERIALSANDMethods:ThisstudywasahospitalbasedstudycarriedoutinLagos,South-westernNigeria.Two hundredpregnantwomenclinicallydiagnosedwithpre-eclampsia(studygroup)and200apparentlyhealthy non-pre-eclampticpregnantwomen(controlgroup)wererecruitedforthestudyafterwritteninformedconsent.Pre-eclampsiawasdiagnosedbasedontheInternationalSocietyfortheStudyofHypertensioninPregnancyre-classificationof2013.MTHFRC677TandMTRA2756Gpolymorphismsweredeterminedbypolymerasechain reaction-restrictionfragmentlengthpolymorphism(PCR-RFLP)method.Statistical analyzes were performed using SPSS version 23. Hardy-Weinberg distribution were tested with χ2test.Logisticregressionmodelwasusedtoevaluatetherelationshipofvariableswithpre-eclampsia.Avalueof p < 0.05 was considered statisticallysignificant.Results: MTHFR genotype frequencies of CC, CT and TT were 59.8%; 31.2% and 9.0% in study group and 76.6%;22.3% and 1.0% in the control group respectively. MTR A2756G genotype frequencies of AA, AG and GGgenotypes were 71.9%; 20.1% and 8.0% for the study group and 81.5%; 16.4% and 2.1% for the control group.Occurrence of pre-eclampsia was significantly associated with presence of T allele of MTHFR (OR = 1.855;p < 0.05) and G allele of MTR genes (OR = 1.269; p < 0.05), Homozygosity of TG haplotype significantly increased the occurrence of pre-eclampsia among Nigerian women (OR = 2.252; p < 0.05). Population at-tributable risk fraction percent for the T and G alleles were 16.4% and 11.5% respectively. Mean plasma Hcy level was not, however, significantly affected by MTHFR/MTR haplotypes (F = 1.54; p = 0.157).Conclusion: MTHFR C677T and MTR A2756G polymorphisms were associated with pre-eclampsia in a popula-tionofpregnantwomeninLagos,Nigeria
Description
Scholarly articles
Keywords
Pre-eclampsia , methylene tetrahydrofolate reductase gene , Polymorphism , Methionine synthase gene polymorphism , Folate metabolism , Research Subject Categories::MEDICINE
Citation
Osunkalu VO, Taiwo IA, Makwe CC and Quao RA (2020). Methylene tetrahydrofolate reductase and methionine synthase gene polymorphisms as genetic determinants of pre-eclampsia. Pregnancy Hypertension. 20 (2020) 7-13. https://doi.org/10.1016/j.preghy.2020.02.001