Immunological Profiles of Mice Protected from Chlamydia-induced Infertility by Anti-caspase Treatment
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Date
2016-04-20
Authors
Ukwade, C.E.
Ebuehi, O.A.T.
Igietseme, J.U.
Ouburg, S.
Land, J.A.
Omosun, Y.
Joseph, K.
Partin, J.
He, Q.
Eko, F.O.
Journal Title
Journal ISSN
Volume Title
Publisher
SCIENCEDOMAIN international
Abstract
The study is to investigate the effect of anti-caspase treatment on anti-chlamydia immune response
in mice. Both the humoral and aspects of cell-mediated immune response against Chlamydia
trachomatis were studied. Antibody response was measured using the ELISA technique to identify
all common isotypes, and cytokine response was measured using the PCR technique. The antibody
levels (IgG, IgG1, IgG2a and IgA) in Z-VAD-FMK treated group were significantly higher than nontreated
group. ELISA results [showed a significantly higher amount of antibodies (IgG, IgG1, Ig G2a and IgA)] were produced in the mice that were pre-treated with Z-VAD-FMK before infection with
Chlamydia trachomatis compared to mice post treated with Z-VAD-FMK after Chlamydia
trachomatis infection. Data of the study indicate that the caspase inhibitor, Z-VAD-FMK did not
negatively affect humoral and T cell mediated immune responses against C. trachomatis in mice.
Description
Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , Chlamydia trachomatis , anti-caspase treatment , immune response , infertility , mice
Citation
Ukwade, C & Ebuehi, Osaretin & Igietseme, Joseph & Ouburg, Sander & Land, J & Omosun, Y & Joseph, K & Partin, James & He, Q & Eko, Francis & Ellerson, D & Bandea, Claudiu & Morré, Servaas & Zhong, G & Black, Carolyn. (2016). Immunological Profiles of Mice Protected from Chlamydia-induced Infertility by Anti-caspase Treatment. British Journal of Medicine and Medical Research. 15. 1-9. 10.9734/BJMMR/2016/23921.