Biochemistry-Scholarly Publications

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Browsing Biochemistry-Scholarly Publications by Author "Adeboyejo, F.O."

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    Open Access
    Anti-diabetic and hypoglycemic properties of fibre-enriched cake in alloxan-induced diabetic rats.
    (Mediterranean Journal of Nutrition and Metabolism, 2013) Erukainure, O.L.; Ebuehi, O.A.T.; Adeboyejo, F.O.; Okafor, E.N.; Hafizur, R.M.; Elemo, G.N
    Studies have provided evidence of the hypoglycemic properties of fiber-containing foods compared to fiber-free foods. In this study, the hypoglycemic properties and beneficial effects of fiber-enriched cake in alloxaninduced diabetic rats were reported. Diabetes was induced by a single intraperitoneal injection of alloxan. Fiberenriched cake was produced from selected fruits and fed to the treatment group. The rats were monitored daily for food intake, and body weight. Their blood glucose levels were monitored on weekly basis. Treatment lasted for 14 days, after which the rats were sacrificed by cervical dislocation. Blood was collected by cardiac puncture and centrifuged to obtain serum which was analyzed to evaluate serum insulin level. Histological study was carried out on the pancreatic tissues. There was no significant difference in diet intake and body weight among the experimental groups. Feeding on the formulated cake led to 68.75 % reduction of the blood glucose level compared to the diabetic group. Significant increase (p\0.05) in serum insulin level was observed in the cake-fed group. Feeding on the formulated cake led to restoration of pancreatic b-cells. The fiberenriched cake had a therapeutic protective effect against diabetes which can serve as a functional food in the treatment and management of the disease.
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    Open Access
    Ebuehi, O.A.T.,Erukainure, O.L.,Adeboyejo, F.O.,Elemo, G.N. Modulation of lipid peroxidation, hypolipidemic and antioxidant activities in brain tissues of diabetic rats by fibre - Enriched biscuits
    (Journal of Acute Disease, 2012) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.
    Objective: To investigate the effect of feeding fibre - enriched biscuit on the antioxidant and hypolipidemic activities in brain tissues of diabetic rats. Method: Diabetes was induced by a single intraperitoneal injection of alloxan. Treatment lasted for 14 d, after which the rats were sacrificed by cervical dislocation. Brain tissues were used for the assessment of GSH, catalase, SOD and lipid peroxidation as well as lipid profiles. Result: Induction of diabetes led to a significant decrease in GSH level, elevated SOD and catalase activities. These were significantly modified by the biscuits. There was an elevated level of malondialdehyde in the brain tissues of the untreated diabetic rats; this was significantly reduced by the biscuits. There was a significant decrease in HDL and a significant increase in LDL levels, total cholesterol and triglycerides in the untreated (diabetic) rats. Feeding with fibre - enriched biscuits led to decrease in the levels of total cholesterol, triglyceride, LDL - cholesterol and caused a significant increase in the levels of HDL. Conclusions: These results suggest a therapeutic and protective effect of the fibre - enriched biscuits against diabetic - induced brain toxicity in rats.
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    Open Access
    Fibre-enriched Cake Attenuates Lipid Peroxidation, Hyperlipidemic and Modulates Redox Activities in Renal Tissues of Diabetic Rats.
    (Pharmacologia, 2013) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.; Aliyu, M.; Nwachukwu, V.A.
    Background: Oxidative stress has been demonstrated in several studies to play a major role in diabetic complications and pathophysiology including nephropathy. The aim ofthis study is to investigate the protective effect of feeding fibre-enriched cake on diabetes-induced oxidative stress and hypercholesterolemia in renal tissues of rats. Materials and Methods: Diabetes was induced by a single intraperitoneal ilkjection of alloxan. Treatment lasted for 14 days, after which the rats were sacrificed by cervical dislocation. Renal tissues were used for the assessment of glutathione (GSH), catalase, superoxide dismutase (SOD) activities, protein content and lipid peroxidation as well as lipid profiles. Results: Induction of diabetes led to significant reduction (p
  • Item
    Open Access
    Modulation of lipid peroxidation, hypolipidemic and antioxidant activities in brain tissues of diabetic rats by fibre - Enriched biscuits
    (Journal of Acute Disease, 2012) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Elemo, G.N.
    Objective: To investigate the effect of feeding fibre - enriched biscuit on the antioxidant and hypolipidemic activities in brain tissues of diabetic rats. Method: Diabetes was induced by a single intraperitoneal injection of alloxan. Treatment lasted for 14 d, after which the rats were sacrificed by cervical dislocation. Brain tissues were used for the assessment of GSH, catalase, SOD and lipid peroxidation as well as lipid profiles. Result: Induction of diabetes led to a significant decrease in GSH level, elevated SOD and catalase activities. These were significantly modified by the biscuits. There was an elevated level of malondialdehyde in the brain tissues of the untreated diabetic rats; this was significantly reduced by the biscuits. There was a significant decrease in HDL and a significant increase in LDL levels, total cholesterol and triglycerides in the untreated (diabetic) rats. Feeding with fibre - enriched biscuits led to decrease in the levels of total cholesterol, triglyceride, LDL - cholesterol and caused a significant increase in the levels of HDL. Conclusions: These results suggest a therapeutic and protective effect of the fibre - enriched biscuits against diabetic - induced brain toxicity in rats.
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    Open Access
    Modulatory effect of fibre-enriched cake on alloxan-induced diabetic toxicity in rat brain tissues.
    (Toxicology Reports(Elsevier), 2014) Ebuehi, O.A.T.; Erukainure, O.L.; Adeboyejo, F.O.; Muhammad Aliyu; Elemo, G.N.
    Diabetes is a metabolic disorder characterized by hyperglycaemia and it is fast becoming a scourge in sub-Saharan Africa. The nutritional properties of developed fibre-enriched cake and its protective potential against diabetic induced neurotoxicity in rats were investigated. Fibre-enriched cake was developed from selected fruits and analysed for its nutritional and sensory attributes. Rats were induced with diabetes by a single intraperitoneal injection of alloxan and treated with the formulated cake. After 14 days treatment, the rats were sacrificed by cervical dislocation. Their brain tissues were accessed for reduced glutathione (GSH), catalase, superoxide dismutase (SOD) activities, protein content and lipid peroxida tion as well as lipid profiles which cover for total cholesterol, triglycerides, HDL and LDL. Induction of diabetes led to significant reduction (p < 0.05) of GSH, catalase, SOD activi ties and protein content. Feeding on the formulated cake led to their significant increase. Decreased lipid peroxidation, total cholesterol, LDL and triglycerides, and increased con centration of HDL were also observed on feeding with the cake. These results indicate an antioxidant protective potential of the fibre-enriched cake against diabetic-induced brain toxicity. Thus, it can serve as an adjunct to dietary therapy for diabetes.
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    Open Access
    Short-Term Feeding of Fibre-Enriched Biscuits: Protective Effect against Hepatotoxicity in Diabetic Rats
    (Hindawi Publishing Corporation, 2015) Erukainure, O.L.; Ebuehi, O.A.T.; Adeboyejo, F.O.; Oladunmoye, O.O.; Aliyu, M; Obode, O.C.; Olasehinde, T.; Elemo, G.N.
    The effects of fibre-enriched biscuit on biomarkers associated with hepatotoxicity in diabetic rats were investigated. Diabetes was induced by single intraperitoneal injection of alloxan monohydrate. Treatment lasted for 14 days after which the rats were sacrificed by cervical dislocation. Blood serumwas analyzed to determine hepatic function enzymes.Theliver was also analyzed to determine hepatic lipid profile and antioxidant enzymes. Induction of diabetes led to elevated levels of ALP, AST, andALT.Thesewere, however, significantly (𝑝 < 0.05) reduced in the fibre-enriched biscuit fed (treated) group.There was no significant difference in the serum bilirubin and total protein levels of the studied groups. Reduced albumin level was observed in the diabetic group; this was further lowered on feeding with fibre-enriched biscuits. Induction of diabetes led to increased hepatic level of cholesterol, triglyceride (TG), low density lipoprotein (LDL), and lipid peroxidation and decreased activities of glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) and HDL level. These were significantly (𝑝 < 0.05) reversed on feeding with fibre-enriched biscuit. This study portrays the protective effect of fibre-enriched biscuit on increased oxidative stress and hyperlipidemia in hepatic tissues of alloxan-induced diabetic rats.